General Histo-technique


Histotechnology Tutorials
If you are unfamiliar with the practice of histotechnology, this is an excellent place to start.  This series of pages includes a tutorial on general Histotechnique(fixation, tissue processing, sectioning, general staining and slide making information), Special Stains(listed by what types of molecules/tissues that they stain) and examples from their Procedure manuals(note: you will need the free Adobe Acrobat reader to view the procedure manuals).  (WebPath, University of Utah, College of Medicine)Histopathology Laboratory Protocols
This site has a wealth of technical information about histologic technique.(The University of Nottingham and the Queens Medical Centre, United Kingdom).Histologic Special Stains Using a Microwave

This page written by Joyce Moore, of the Jefferson Regional Medical Center Histo-Path Laboratories, outlines the use of Microwaves in histologic staining.  Other interesting pages at this site (WWW site courtesy of EBSciences):  Microwave Antigen Retrieval Technique, Working Procedures in Microwave Histology and Microwave Processing Techniques for Microscopy & Histology.Laboratory Protocols

An excellent resource with information about: cryostat sectioning, histologic fixatives, histologic stains, peroxidase techniques, treating slides for "stickiness", tissue processing and specimen collection(Veterinary Pathology, University of Bristol, UK).Protocols On-Line - Immunology>Histological Techniques

StainsFile: A Resource for Histotechnologists

This site, developed by Bryan D. Llewellyn, is primarily about histologic staining techniques and dyes.The Histology Page

Includes extracts from Woods and Ellis, "Laboratory Histopathology: A Complete Reference" (1994 Churchill Livingstone). Topics include: Cryotechniques, Fixation, Haematoxylin and Counterstains, Microtomy, Mountants, Resin Processing and Tissue Processing.Techniques Manual